Kodi umuna cryopreservation

Pakuti ubwamuna m'thupi mwake chotchuka umuna cryopreservation. Ndi yaitali yosungirako umuna wosalira kuchititsa lidzawagwiritsenso. The ntchito ndi umuna ntchito amakhalabe chifukwa kutentha otsika - yozizira ndi asafe. Nzeru kusunga njira amalola kuti abwezeretse tizilombo wamoyo, ngakhale zaka khumi kenako.

N'chifukwa chiyani ndikufunika kuzizira zotsatira

Umuna cryopreservation akulimbikitsidwa anthu monga njira yothetsera vuto osabereka. njira amakhala yankho kwa akazi amene safuna kukhudzana ndi munthu wodwala. Yozizira chenicheni pamene pali chiopsezo cha HIV, koma palibe kuopseza mwana osabadwa.

Amuna angalakwe m'tsogolo pamene chiopsezo alipo thanzi:

• zosokonezeka matenda aakulu a dongosolo urogenital;
• zotsogola Oncology;
• adzaimira ntchito pambuyo choipa;
• kulemala.

Kusunga zinthu zamoyo, ndipo anthu ali ofunitsitsa adziwitse opaleshoni, cheza kapena mankhwala amphamvu. Komanso, anthu kusankha njira zasayansi kuti kuzizira mkazi wa inshuwalansi, kupita ku ntchito ndi chiopsezo moyo.

vitrification njira kusunga zipangizo kwachilengedwenso:

• umuna;
• mazira;
• maselo ofiira;
• ziwalo;
• dzira.

Umuna Cryopreservation mu Moscow ayamba kutchuka chifukwa kupezeka. Koposa zipatala kupereka ntchito kusunga zipangizo kwachilengedwenso. Anthu ndi kuyamba kugwiritsa ntchito njirayi ngati njira yopezera ndalama, anapereka thanzi labwino kwa thupi. Othandizira ndalama ndi zofunika akazi ndi mabanja omwe akudwala osabereka.

njira ndizosowa

Kuteteza maselo ku chiwonongeko chinthu chapadera - cryoprotectant. amaundana zingakhale bwino popanda kugwiritsa ntchito chinthu.

Koma pa zamoyo pamene anachita umuna cryopreservation, mphamvu ndi zinthu zoipa:

• mapangidwe timibulu ayezi mwa maselo a khungu madzi;
• monga chotsatira cha chinthu choyamba pali madzi m'thupi.

Kungopeweratu kuwonongedwa kwa maselo amoyo, iwo anayikidwa mu njira cryoprotectant. chitetezo zinthu anawagawira ndi cholinga:

• ozama - akuletsa crystallization madzi mkati maselo;
• nonpenetrating - ndi wowonjezera okha yoyamba cha chitetezo chimene ndikulinga kuti akalandire selo kukhala zinthu zoipa kunja.

Gulu loyamba zikuphatikizapo: ethylene glycol, dimethyl sulfoxide, propylene glycol. zinthu zina ndi: sucrose, ficoll. N'zosiyana ndondomeko defrosting limodzi ndi mulinso kutulutsidwa kwa maselo a kugwirizana ndi cryoprotectants.

Kukonzekera ndi wozengereza njira

Pamaso cryopreservation umuna ikuchitika, izo ikuchitika kufufuza zonse za boma la woperekayo thupi. Wounikira chachikulu cha kusanthula umuna chimakhala spermogram. chipatala anachita mayeso mlandu wa zinthu yozizira koopsa munthu. Ndi chofunika kuti ayese luso la mbewu kuti achire yosungirako yaitali.

Otchedwa kriotest amathandiza madokotala amayang'anitsitsa chiphaso cha chitsimikizo cha umuna wathanzi. mayesero zina ukunenedwa kuteteza mwana wosabadwayo ndi mayi ake ku matenda:

• nsungu simplex HIV;
• Chiwindi B ndi C;
• matenda HIV;
• chindoko.

Anapempha makasitomala angathe kuchitidwa pa Kupenda zonse za mwiniwake. mayesero imachitika Mphindi 20 pambuyo ndondomeko thawing. Ngati macheke onse ndi zinthu zabwino, dontho la umuna ali osokonezeka ndi njira ya cryoprotectants ndipo anaikidwa m'dera wapadera yosungirako.

Kodi kusunga biomaterial

Mu muli kumene kukonzedwa madzimadzi umuna, ayenera anakhalabe kutentha -196 ° C. Kukwaniritsa zinthu oyenera kugwiritsa ntchito zinthu zenizeni - asafe.

Anthu umuna Cryopreservation imagwiridwa machubu polypropylene (Dewar ziwiya), mmene biomaterial ndi kusungidwa. The kapisozi chomwecho aikidwa mu chidebe ndi asafe.

yosungirako yaitali ndi zaka 3, pa nthawi mgwirizanowo wopangidwa ndi woperekayo. Chakuti kayendedwe ka muli ndi zipatala zina mwa kuchenjera kwa makasitomala. Izi amafuna kutsimikizira olembedwa choti kunyamula umuna mazira. The wodwala anapereka pepala pa nthawi ya Nsanja ya biomaterial lapansi.

The momwe akadakwanitsira alumali moyo wa ejaculate, wofanana zaka zitatu. Timeout sizitanthauza kuteteza khalidwe la zinthu zamoyo, popeza ndondomeko ya kusintha mkati mwa maselo akuyamba. Medicine komabe alibe mphamvu ya kalekale amaundana, kotero woperekayo amalingalira ngati kusunga umuna mu beseni, kapena kubwereza ndondomeko atetezedwe.

Kodi mtengo wa utumiki zimatengera

Kuwanda kwa maganizo kuchokera ku zipatala payekha aukitsa vuto kusankha bungwe woonamtima, kuchititsa ndondomeko Mkhalidwe. Zipatala akhoza kugwiritsa ntchito njira miyambo ya yozizira koopsa - yapang'onopang'ono umuna cryopreservation. Mtengo wa utumiki uwu osiyana pang'ono, koma pali mavuto kuwonongeka kwa ejaculate. Wamba njira vitrification - ndondomeko superfast kuteteza biomaterial.

The ndalama pafupifupi misonkhano anafotokoza mu dongosolo la 10 400 rubles. ndipo zimasiyanasiyana malinga zifukwa zotsatirazi:

• Clinic kutchuka, chiwerengero cha usilikali ndi ndemanga zabwino;
• cryopreservation njira (pang'onopang'ono yozizira koopsa kapena vitrification);
• tsiku lomalizira kusaina mgwirizano - malipiro a mtengo wa nthawi yaitali kwambiri yafupika;
• zinthu yosungirako (ambiri mosungiramo kapena chidebe osiyana).

Pakuti kusungiramo mazira kwa mwezi 1 kulipira rubles 1000., Ndipo nkhani za yosungirako chipinda otedlnoe kwa nthawi yomweyi ndalama 4000 rubles.

Mbiri ya yatsoka njira yozizira koopsa

Yonena za ndondomeko zinachitika pambuyo kumayambiriro bwino maselo ndi kubadwa kwa mwana wathanzi mu Netherlands. zochitika tsiku kumbuyo kwa chaka cha 1984, pamene cryopreservation la umuna wakhala bwinobwino kuyesedwa. Technology lidakalipobe kwa nthawi yathu, koma pakhala patsogolo kwambiri kuzizira ndondomeko.

Amapanga insemination wa lero amachititsa maganizo anthu. funso n'lakuti:

• Kalembera wa mwalamulo mwana, kukhazikitsidwa kwa umayi ndi bambo;
• kusungidwa koyenela mbali lingaliro zobwera njira;
• anthu ena magulu kum'mawa sali okonzekera njira yatsopano ya moyo.

Koma ngakhale mavuto, anthu akugwiritsa ntchito cryopreservation monga njira yosungira thanzi la mwanayo tsogolo.